|
Primary Antibodies
SOCS1 Antibody
Background
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines (1). SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase (2). SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome (3). It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP (4).
Additional Names
SOCS1 (CT), Suppressor of cytokine signaling 1, JAK-binding protein, JAB
|
Description
Left: Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 μg/ml.
|
Source
SOCS1 antibody was raised against an 15 amino acid peptide from near the carboxy terminus of human SOCS1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
SOCS1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human SOCS1 (C-Terminus) Peptide (Cat. No. 3765P)
Application
SOCS1 antibody can be used for the detection of SOCS1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3765P - SOCS1 Peptide
Storage
SOCS1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
8217331
Protein Accession Number
CAB92528
Short Description
(CT) Suppressor of cytokine signaling 1
This product belongs to the following categories:
Related Products
References- Rakesh K and Agrawal DK. Controlling cytokine signaling by constitutive inhibitors. Biochem. Pharm. 2005; 70:649-57.
- O’Shea JJ, Gadina M, and Schreiber RD. Cytokine signaling in 2002: new surprises in the Jak/Stat pathway. Cell 2002; 109:S121-31.
- Kile BT, Schulman BA, Alexander WS, et al. The SOCS box: a tale of destruction and degradation. Trends Biochem. Sci. 2002; 27:235-41.
- Mansell A, Smith R, Doyle SL, et al. Suppressor of cytokine signaling 1 negatively mediates Toll-like receptor signaling by mediating Mal degradation. Nat. Immunol. 2006; 7:148-55.
Datasheet 08-02W
|
|
