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Primary Antibodies
SMAD6 Antibody
Background
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
Additional Names
SMAD6
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Description
Left: Western blot analysis of SMAD6 in Jurkat Cell Lysate using SMAD6 antibody at 2 μg/ml (12% gel).
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Purification
Antibody is supplied as total IgG.
Clonality / Clone
This is a polyclonal antibody.
Host
SMAD6 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD6.
Application
SMAD6 antibody can be used for detection of SMAD6 by ELISA at 1:62500. SMAD6 antibody can be used for detection of SMAD6 by western blot at 0.5-1.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB, IHC
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store SMAD6 antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, M, R, D
Protein Accession Number
NP_005576
Molecular Weight
53 kDa
Short Description
SMAD6 (SMAD family member 6)
This product belongs to the following categories:
References- Tsunobuchi,H., et al., (2004) Biophys. Res. Commun. 316(3),712-719.
Datasheet 10-01W
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