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Primary Antibodies
SMAD6 Antibody
Background
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used asalternate namesfor one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1.
Additional Names
SMAD6, HSMAD6, HST17432, MADH6, MADH7, smad6
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Description
Left: Immunohistochemistry staining of SMAD6 in testis (formalin-fixed paraffin-embedded) using SMAD6 antibody.
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Purification
Protein G Column
Clonality / Clone
This is a polyclonal antibody.
Isotype
IgG
Host
SMAD6 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
SMAD6 antibody was raised against synthetic peptides corresponding to amino acids 85-99 of SMAD6. (Human)
Application
SMAD6 antibody can be used for detection of SMAD6 by WB (5 - 2 μg/ml), IHC-parafin (5 μg/ml).
Tested Application
WB, IHC
Buffer
SMAD6 antibody is supplied in PBS, 0.05% BSA, 0.05% sodium azide.
Storage
SMAD6 antibody can be stored at 4˚C for short term storage, for long term storage aliquot and store at -20˚C . Avoid freeze thaw cycles.
Species Reactivity
M, R, Mk
Formulation
Weight: 50 μg
Concentration: 0.5 mg/ml
Short Description
SMAD
This product belongs to the following categories:
Datasheet 09-01W
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