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Primary Antibodies
SMAD3 Antibody
Background
Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Additional Names
SMAD3, DKFZP586N0721, DKFZP686J10186, HMAD-3, HSMAD3, HSPC193, HST17436, JV15-2, MAD3, MADH3, MGC60396, mad homolog jv15-2, Mothers Against Decapentaplegic Homolog 3
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Description
Left: Immunohistochemistry staining of SMAD3 in skin (formalin-fixed paraffin-embedded) using SMAD3 antibody.
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Purification
Immunoaffinity Chromatography
Clonality / Clone
This is a polyclonal antibody.
Host
SMAD3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Specificity
pSer423/425
Immunogen
SMAD3 antibody was raised against amino acids 417-425 of SMAD3. (Human)
Application
SMAD3 antibody can be used for detection of SMAD3 by WB (1:2000 - 1:20000), ELISA (1:25000 - 1:100000), IF, IHC-parafin (2.5 μg/ml).
Tested Application
E, WB, IHC, IF
Buffer
SMAD3 antibody is supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
SMAD3 antibody can be stored at 4˚C or -20˚C, Avoid repeated freezing and thawing.
Species Reactivity
M, R, B, P, C, X, ZF
Formulation
Weight: 50 μg
Volume: 50 μl
Concentration: 1 mg/ml
Short Description
SMAD
This product belongs to the following categories:
Datasheet 09-01W
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