|
Primary Antibodies
SMAD3 Antibody
Background
Principal Names: Mothers against Decapentaplegic homolog 3 (Drosophila); SMAD3; hMAD-3; hSMAD3; HSPC193; JV15-2; MADH3; MGC60396; Official Gene Symbol: SMAD3 Gen Bank Accession Number: NP_005893 Gene ID: 4088 (Human) Gene Map Locus: 15q22.33 (Human) SMAD3, a member of SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia. Smad 3 (Mothers against decapentaplegic homolog 3), a transcriptional regulator, is activated by TGF-beta and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC. Upon phosphorylation at two extreme C-terminal serines, SMAD3 is translocated into the nucleus where is forms complexes other SMAD proteins, depending on the activation pathway, and regulates the transcription of target genes.
Additional Names
SMAD3, DKFZP586N0721, DKFZP686J10186, HMAD-3, HSMAD3, HSPC193, HST17436, JV15-2, MAD3, MADH3, MGC60396, mad homolog jv15-2, Mothers Against Decapentaplegic Homolog 3
|
Description
Left: Immunohistochemistry staining of SMAD3 in kidney (formalin-fixed paraffin-embedded) using SMAD3 antibody.
|
Purification
Protein G Column
Clonality / Clone
This is a polyclonal antibody.
Host
SMAD3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
SMAD3 antibody was raised against a portion of amino acids 100-150 of SMAD3. (Human)
Application
SMAD3 antibody can be used for detection of SMAD3 by WB (5 - 2 μg/ml), IHC-parafin (10 μg/ml).
Tested Application
WB, IHC
Buffer
SMAD3 antibody is supplied in PBS, 0.2% gelatin, 0.05% sodium azide.
Storage
SMAD3 antibody can be stored at 4˚C for short term storage, for long term storage aliquot and store at -20˚C . Avoid freeze thaw cycles.
Species Reactivity
M, R, B, P, Mk
Formulation
Weight: 50 μg
Concentration: 0.5 mg/ml
Short Description
SMAD
This product belongs to the following categories:
Datasheet 09-01W
|
|
