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Primary Antibodies
SIPA1L2 Antibody
Background
Signal-induced proliferation associated-like protein 2 (SIPA1L2) is a member of the SIPA1 family of RapGAPs. Little is known of the role of the SIPA1L2 protein, but recent studies of SIPA indicate that its deregulation can cause myeloproliferative stem cell disorders in mice and increased metastases in human cancers. Other studies suggest SIPA1L1 may play important roles in embryo development and control of cell proliferation. Based on the amount of homology between SIPA family members, it is likely that SIPA1L2 plays a role in embryo development and cell proliferation, possibly including oncogenesis.
Additional Names
SIPA1L2, Signal-induced proliferation associated-like protein 2, SPAL2
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Description
Left: Western blot analysis of SIPA1L2 in rat brain tissue lysate with SIPA1L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Below: Immunohistochemistry of SIPA1L2 in human brain tissue with SIPA1L2 antibody at 5 μg/ml.
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Other Product Images
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Source
SIPA1L2 antibody was raised against a 19 amino acid peptide from near the amino terminus of human SIPA1L2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
SIPA1L2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
SIPA1L2 antibody can be used for detection of SIPA1L2 by Western blot at 0.5 - 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5493P - SIPA1L2 Peptide
Storage
SIPA1L2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
112421013
Protein Accession Number
NP_065859
Short Description
Signal-induced proliferation associated-like protein 2
This product belongs to the following categories:
References- Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci. 2009; 100:17-23.
- Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell 2003; 4:55-65.
- Park YG, Zhao X, Lesueur F, et al. Sipa1 is a candidate for underlying the metastasis efficiency modifier locus, Mtes. Nat. Genet. 2005; 37:1055-62.
- Tsai I-C, Amack JD, Gao Z-H, et al. A Wnt-CKI e-Rap1 pathway regulates gastrulation by modulating SIPA1L2, a Rap GTPase activating protein. Dev. Cell 2007; 12:335-47.
Datasheet 10-01W
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