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Primary Antibodies
SHOC2 Antibody
Background
SHOC2 protein participates in protein binding / transferase activity in the fibroblast growth factor receptor signaling pathway and Ras protein signal transduction. It is a widely expressed protein composed almost entirely of leucine-rich repeats (LRR), with a lysine-rich sequence at the amino terminus and cytoplasmically localized. SHOC2 acts as a positive modulator of the RAS-MAPK signaling cascade, which is elicited by EGL-15 and LET-23 and mediated by LET-60. SHOC2 together with protein phosphatase 1c (PP1c) forms a highly specific M-Ras effector complex and is essential for activation of the MAPK pathway by growth factors. Furthermore, in tumor cells with Ras gene mutations, inhibition of SHOC2 expression inhibits MAPK, but not PI3K activity. The SHOC2-PP1c holoenzyme provides an attractive therapeutic target for inhibition of the MAPK pathway in cancer. Recent studies show that aberrantly acquired N-myristoylation of SHOC2 causes human disease Noonan-like syndrome with loose anagen hair.
Additional Names
SHOC2, Leucine-rich repeat protein SHOC-2, Ras-binding protein Sur-8, SOC2
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Description
Left: Western blot analysis of SHOC2 in Jurkat cell lysate with SHOC2 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of SHOC2 in human spleen tissue with SHOC2 antibody at 5 μg/ml.
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Other Product Images
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Source
SHOC2 antibody was raised against a 21 amino acid peptide near the amino terminus of human SHOC2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
SHOC2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
SHOC2 antibody can be used for detection of Shoc2 by Western blot at 1-2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5215P - SHOC2 Peptide
Storage
SHOC2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
41281398
Protein Accession Number
NP_031399
Short Description
Ras-binding protein Sur-8
This product belongs to the following categories:
References- Selfors LM, Schutzman JL, Borland CZ, et al. Soc-2 encodes a leucine-rich repeat protein implicated in fibroblast growth factor receptor signaling. Proc. Natl. Acad. Sci. USA 1998; 95:6903-8.
- Sieburth DS, Sun Q, and Han M. SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans. Cell 1998; 94:119–130.
- Rodriguez-Viciana P, Oses-Prieto J, Burlingame A, et al. A phosphatase holoenzyme comprised of Shoc2/Sur8 and the catalytic subunit of PP1 functions as an M-Ras effector to modulate Raf activity. Mol. Cell 2006; 22:217-30.
- Li W, Han M, and Guan KL. The leucine-rich repeat protein SUR-8 enhances MAP kinase activation and forms a complex with Ras and Raf. Genes Dev. 2000; 14:895–900.
Datasheet 09-01W
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