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Primary Antibodies
SGK Antibody
Background
SGK-1 (also called Serine/threonine-protein kinase Sgk1 and Serum/glucocorticoid-regulated kinase 1) is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. This kinase mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A and phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1. SGK1 is localized to the cytoplasm and upon phosphorylation is translocated to the nucleus. The kinase is expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Induction occurs upon exposure to glucocorticoids and by excessive extracellular glucose or TGF-beta, in cultured cells. SGK-1 is regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. The kinase is ubiquitinated by NEDD4L; which promotes proteasomal degradation.
Additional Names
SGK1, hsgk, hsgk1, serine/threonine protein kinase sgk, Serum- and glucocorticoid-inducible kinase, serum/glucocorticoid regulated kinase , sgk, sgk1
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Description
Left: Immunohistochemistry staining of SGK in pancreas (formalin-fixed paraffin-embedded) using SGK antibody.
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Purification
Immunoaffinity Chromatography
Clonality / Clone
This is a polyclonal antibody.
Host
SGK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
SGK antibody was raised against amino acids 419-431 of SGK. (Human)
Application
SGK antibody can be used for detection of SGK by WB (1:500 - 1:3000), ELISA (1:4000 - 1:16000), IHC-parafin (10 - 20 μg/ml).
Tested Application
E, WB, IHC
Buffer
SGK antibody is supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
SGK antibody can be stored at 4˚C or -20˚C, Avoid repeated freezing and thawing.
Species Reactivity
H
Formulation
Weight: 50 μg
Volume: 50 μl
Concentration: 1 mg/ml
Short Description
Serum/Glucocorticoid Regulated Kinase 1
This product belongs to the following categories:
Datasheet 09-01W
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