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Primary Antibodies
SCF Antibody
Background
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-g, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
Additional Names
SCF, Stem cell factor, Mast cell growth factor, MGF, c-kit ligand, KL-1, KITLG, SHEP7
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Description
Left: Western blot analysis of SCF in rat brain tissue lysate with SCF antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of SCF in mouse brain tissue with SCF antibody at 2.5 μg/ml.
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Other Product Images
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Source
SCF antibody was raised against an 18 amino acid peptide from near the center of human SCF.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
SCF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
SCF antibody can be used for detection of SCF by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5165P - SCF Peptide
Storage
SCF antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
134289
Protein Accession Number
P21583
Short Description
Stem cell factor
This product belongs to the following categories:
References- Reber L, Da Silva CA, and Frossard N. Stem cell factor and its receptor c-Kit as targets for inflammatory diseases. Euro. J. Pharmacology 2006; 533:327-40.
- Jensen BM, Metcalfe DD, and Gilfillan AM. Targeting kit activation: a potential therapeutic approach in the treatment of allergic inflammation. Inflamm. Allergy Drug Targets 2007; 6:57-62.
- Lukacs NW, Strieter RM, Lincoln PM, et al. Stem cell factor (c-kit ligand) influences eosinophil recruitment and histamine levels in allergic airway inflammation. J. Immunol. 1996; 156:3945-51.
Datasheet 09-01W
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