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Primary Antibodies
SARS Spike Antibody
Background
A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2 (4).
Additional Names
SARS Spike (NT), SARS
Source
SARS Spike antibody was raised against a synthetic peptide corresponding to amino acids at the amino-terminus of the SARS Spike glycoprotein.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
SARS Spike antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human SARS Spike (N-Terminus) Peptide (Cat. No. 3219P)
Application
SARS Spike antibody can be used for the detection of SARS Spike protein in ELISA. It will detect 10 ng of free peptide at 1 μg/ml. Other applications are pending.
Tested Application
E
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3219P - SARS Spike Peptide
Storage
SARS Spike antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
V
Protein GI Number
30173397
Protein Accession Number
P59594
Short Description
(NT) SARS virus membrane protein
This product belongs to the following categories:
Related Products
References- Marra MA, Jones SJ, Astell CR, et al. The Genome sequence of the SARS-associated corona virus. Science 2003;300:1399-404.
- Rota PA, Oberste MS, Monroe SS, et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 2003;300:1394-9.
- Navas-Nartin SR and Weiss S. Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development. J Neurovirol. 2004;10:75-85.
- Li W, Moore MJ, Vasileva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003;426:450-4.
Datasheet 08-02W
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