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Primary Antibodies
Rim2 Antibody
Background
Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner. Rim2 is known to exist in multiple isoforms; this antibody should recognize most of them. This antibody is predicted to have no cross-reactivity to other Rim proteins.
Additional Names
Rim2, Rab3-interacting molecule 2, regulating synaptic membrane exocytosis 2, RIMS2
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Description
Left: Western blot analysis of Rim2 in rat brain tissue lysate with Rim2 antibody at 1 μg/ml in the (A) absence or (B) presence of blocking peptide.
Below: Immunohistochemistry of Rim2 in human brain with Rim2 antibody at 5 μg/ml.
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Other Product Images
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Source
Rim2 antibody was raised against a 17 amino acid peptide near the center of the human Rim2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Rim2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Rim2 antibody can be used for detection of Rim2 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4609P - Rim2 Peptide
Storage
Rim2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
154354983
Protein Accession Number
NP_001093587
Short Description
Rab3-interacting molecule 2
This product belongs to the following categories:
Related Products
References- Wang Y, Sugita S, and Sudhof TC. The RIM/NIM family of neuronal C2 domain proteins: interactions with Rab3 and a new class of Src homology 3 domain proteins. J. Biol. Chem. 2000; 275:20033-44.
- Liang F, Zhang B, Tang J, et al. RIM3gamma is a postsynaptic protein in the rat central nervous system. J. Comp. Neurol. 2007; 503:501-10.
- Shoch S, Mittelstaedt T, Kaeser PS, et al. Redundant functions of RIM1a and RIM2a in Ca2+-triggered neurotransmitter release. EMBO J. 2006; 25:5852-63.
Datasheet 08-03W
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