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Primary Antibodies
Rheb Antibody
Background
Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway (3-5). TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2 (3).
Additional Names
Rheb (NT), Rheb
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Description
Left: Western blot analysis of Rheb in rat heart cell lysate with Rheb antibody at (A) 2 and (B) 4 μg/ml.
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Source
Rabbit Rheb polyclonal antibody was raised against a 14 amino acid peptide from the amino terminus of human Rheb.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Rheb antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Rheb (N-Terminus) Peptide (Cat. No. 3499P)
Application
Rheb antibody can be used for the detection of Rheb by Western blot at 2 - 4 μg/ml. Post-translationally modified Rheb is sometimes observed at higher molecular weight.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3499P - Rheb Peptide
Storage
Rheb antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1461 - Rat Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
16740561
Protein Accession Number
AAH16155
Short Description
(NT) Ras homolog enriched in brain
This product belongs to the following categories:
Related Products
References- Yamagata K, Sanders LK, Kaufman WE, et al. rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein. J. Biol. Chem. 1994; 269:16333-9.
- Yee WM and Worley PF. Rheb interacts with Raf-1 kinase and may function to integrate growth factor- and protein kinase A-dependent signals. Mol. Cell. Biol. 1997; 17:921-3.
- Inoki K, Li Y, Xu T, et al. Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev. 2003; 17:1829-34.
- Stocker H, Radimerski T, Schindelholz B, et al. Rheb is an essential regulator of S6K in controlling cell growth in Drosophila. Nat. Cell Biol. 2003; 5:559-65.
Datasheet 08-02W
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