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Primary Antibodies
Raptor Antibody
Background
The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation (3). These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels (4). Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins (5).
Additional Names
Raptor (CT), Raptor
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Description
Left: Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 μg/ml.
Below: Immunocytochemistry of Raptor in L1210 cells with Raptor antibody at 10 μg/ml.
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Other Product Images
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Source
Raptor (CT) polyclonal antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of mouse Raptor.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Raptor antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Virus Raptor (C-Terminus) Peptide (Cat. No. 3489P)
Application
Raptor antibody can be used for the detection of Raptor by Western blot at 2 μg/ml. Raptor has multiple isoforms that may also be recognized by antibody.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3489P - Raptor Peptide
Storage
Raptor antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1284 - L1210 Cell Lysate
Species Reactivity
M
Protein GI Number
46577497
Protein Accession Number
Q8K4Q0
Short Description
(CT) a member of the TOR signaling pathway
This product belongs to the following categories:
Related Products
References- Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
- Fingar DC and Blenis J. Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene 2004; 23:3151-71.
- Yonezawa K, Tokunaga C, Oshiro N, et al. Raptor, a binding partner of target of rapamycin. Biochem. Biophys. Res. Commun. 2004; 313:437-441.
- Hara K, Yonezawa K, Weng QP, et al. Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism. J. Biol. Chem. 1998; 273:14484-94.
Datasheet 08-02W
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