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Primary Antibodies
RUNX1 Antibody
Background
RUNX1 is one of three mammalian RUNX genes that control multiple aspects of embryonic development and are responsible for the pathogenesis of many human diseases. RUNX1 plays major roles in the development of nociceptive sensory neurons in addition to hematopoietic stem cells (HSC) with the exception of the erythroid lineage. During development, Notch signals mediate RUNX1 induction with SCL/GATA/Ets factors, and Wnt signals potentially cooperate with RUNX1 to facilitate adult HSC expansion via cooperative induction of cyclin D, cdk4, and other cell cycle regulators. In turn, RUNX1 regulates cell cycle transitions dependent on functional/physical interactions with other proteins such as HDAC1 and -3, mSin3A, p300, SMAD proteins, and LEF/TCF.
Additional Names
RUNX1, Runt-related transcription factor 1, AML1, AMLCR1, CBFA2, EVI-1
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Description
Left: Western blot analysis of RUNX1 in Raji cell lysate with RUNX1 antibody at (A) 1 and (B) 2 μg/ml.
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Source
RUNX1 antibody was raised against a 16 amino acid peptide from near the center of human RUNX1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RUNX1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
RUNX1 antibody can be used for detection of RUNX1 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5149P - RUNX1 Peptide
Storage
RUNX1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1207 - Raji Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
223459612
Protein Accession Number
AAI36381
Short Description
Runt-related transcription factor 1
This product belongs to the following categories:
References- Lund AH and van Lohuizen M. RUNX: a trilogy of cancer genes. Cancer Cell 2002; 1:213-5.
- Inoue K, Shiga T, and Ito Y. Runx transcription factors in neuronal development. Neur. Dev. 2008; 3:20.
- North TE, Stacy T, Matheny CJ, et al. Runx1 is expressed in adult mouse hematopoietic stem cells and differentiating myeloid and lymphoid cells, but not in maturing erythroid cells. Stem Cells 2004; 22:158-68.
- Lee TI, Jenner RG, Boyer LA. Control of developmental regulators by Polycomb in human embryonic stem cells. Cell 2006; 125:301-13.
Datasheet 09-01W
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