|
Primary Antibodies
RPS6K1 Antibody
Background
Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (1,2). Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and G?L to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth (3). RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice (4).
Additional Names
RPS6K1, Ribosomal protein S6 kinase 1, S6K, p70 S6 kinase, p70-S6K
|
Description
Left: Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/ml.
Below: Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
RPS6K1 antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human RPS6K1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RPS6K1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Human RPS6K1 / Ribosomal protein S6 kinase 1 / S6K / p70 S6 kinase / p70-S6K Peptide (Cat. No. 3511P)
Application
RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 5 - 10 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3511P - RPS6K1 Peptide
Storage
RPS6K1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein GI Number
292457
Protein Accession Number
AAC82497
Short Description
A kinase involved in cell growth and metabolism
This product belongs to the following categories:
AntibodiesPolyclonal AntibodiesSignal Transduction Antibodies
Related Products
References- Burnett PE, Barrow RK, Cohen NA, et al. RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1. Proc. Natl. Acad. Sci. USA. 1998; 95:1432-7.
- Inoki K, Ouyang H, Li Y, et al. Signaling by target of rapamycin proteins in cell growth control. Microbiol. Mol. Biol. Rev. 2005; 69:79-100.
- Manning BD. Balancing Akt with S6K: implications for both metabolic diseases and tumorigenesis. J. Cell Biol. 2004; 167:399-403.
- Um SH, Frigerio F, Watanabe M, et al. Absences of S6K1 protects against age- and diet-induced obesity while enhancing insulin sensitivity. Nature 2004; 431:200-5.
Datasheet 08-02W
|
|
