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Primary Antibodies
RIPK1 Antibody
Background
RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell’s decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.
Additional Names
RIPK1, Receptor-interacting serine/threonine-protein kinase 1, RIP, RIP1
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Description
Left: Western blot analysis of RIPK1 in rat kidney tissue lysate with RIPK1 antibody at 1 μg/ml.
Below: Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
RIPK1 antibody was raised against a 15 amino acid peptide from near the amino terminus of human RIPK1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RIPK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
RIPK1 antibody can be used for detection of RIPK1 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5389P - RIPK1 Peptide
Storage
RIPK1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1465 - Rat Kidney Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
57242761
Protein Accession Number
NP_003795
Short Description
Receptor-interacting kinase 1
This product belongs to the following categories:
References- Stanger BZ, Leder P, Lee TH, et al. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell 1995; 81:513-23.
- Hsu H, Huang J, Shu HB, et al. TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. Immunity 1996; 4:387-96.
- Meylan E, Burns K, Hofmann K, et al. RIP1 is an essential mediator of Toll-like receptor 3-induced NF-kappa B activation. Nat. Immunol. 2004; 5:503-7.
- Festjens N, Vanden Bergh T, Cornelis S, et al. RIP1, a kinase on the crossroads of a cell's decision to live or die. Cell Death Differ. 2007;14:400-10.
Datasheet 09-01W
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