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Primary Antibodies
RIP3 Antibody
Background
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 (1-3). RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues (2,3).
Additional Names
RIP3
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Description
Left: Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of blocking peptide with RIP3 antibody at 1 μg/ml.
Below: Immunohistochemistry of RIP3 in rat kidney tissue with RIP3 antibody at 5 μg/ml.
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Other Product Images
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Source
RIP3 antibody was raised against a peptide corresponding to amino acids 473 to 486 of murine RIP3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RIP3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human RIP3 Peptide (Cat. No. 2283P)
Application
RIP3 antibody can be used for detection of RIP3 by Western blot at 0.5 to 1 μg/ml. An approximately 57 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
RIP3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1212 - 3T3 Cell Lysate
Species Reactivity
M, R
Protein GI Number
6063101
Protein Accession Number
AAF03133
This product belongs to the following categories:
Related Products
References- Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that activates apoptosis and NFκB. Curr Biol. 1999;9(10):539-42.
- Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 1999;274(24):16871-5.
- Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102)
Datasheet 08-02W
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