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Primary Antibodies
RIG-1 Antibody
Background
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKε through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.
Additional Names
RIG-1, Retinoic acid inducible gene 1 protein, RIG-I, Dead box protein 58, ddx58
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Description
Left: Western blot analysis of RIG-1 in C2C12 cell lysate with RIG-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of RIG-1 in human heart tissue with RIG-1 antibody at 5 μg/ml.
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Other Product Images
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Source
RIG-1 antibody was raised against human GST-tagged RIG-1 protein.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RIG-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
RIG-1 antibody can be used for the detection of RIG-1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3953P - RIG-1 Peptide
Storage
RIG-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1285 - C2C12 Cell Lysate
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
81170421
Protein Accession Number
O95786
Short Description
RIG-I, dsRNA, TLR3, RNA virus
This product belongs to the following categories:
Related Products
References- Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
- Yoneyama M, Kikuchi M, Natsukawa T, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 2004; 5:730-7.
- Alexopoulou L, Holt AC, Medzhitov R, et al. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 2001; 413:732-8.
- Sharma S, tenOever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
Datasheet 08-02W
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