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Primary Antibodies
RICK Antibody
Background
Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively, (1-3). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues.
Additional Names
RICK (NT), RIP2
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Description
Left: Western blot analysis of RICK in A431 (A) and K562 (K) whole cell lysate with RICK antibody at 1:1000 dilution.
Below: Immunocytochemistry of RICK in A431 cells with RICK antibody at 10 μg/ml.
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Other Product Images
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Source
RICK antibody was raised against a peptide corresponding to amino acids near the amino terminus of human RICK.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RICK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human RICK (N-Terminus) Peptide (Cat. No. 2075P)
Application
RICK antibody can be used for detection of RICK by Western blot at 1:1000 dilution. An approximately 60 kDa band can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2075P - RICK Peptide
Storage
RICK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
20455217
Protein Accession Number
O43353
Short Description
(NT) RIP-like Interacting CLARP Kinase
This product belongs to the following categories:
Related Products
References- Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem 1998;273:12296-300
- McCarthy JV, Ni J, Dixit VM. RIP2 is a novel NF-κB-activating and cell death-inducing kinase. J Biol Chem 1998;273:16968-75
- Thome M, Hofmann K, Burns K, Martinon F, Bodmer JL, Mattmann C, Tschopp J. Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr Biol 1998;8:885-8 (RD1299)
Datasheet 08-02W
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