|
Primary Antibodies
RHBDD1 Antibody
Background
The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.
Additional Names
RHBDD1, Rhomboid domain containing 1
|
Description
Left: Western blot analysis of RHBDD1 in K562 cell lysate with RHBDD1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
|
Source
RHBDD1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human RHBDD1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RHBDD1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
RHBDD1 antibody can be used for detection of RHBDD1 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5525P - RHBDD1 Peptide
Storage
RHBDD1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H, M
Protein GI Number
71680537
Protein Accession Number
AAI01265
Short Description
Rhomboid domain containing 1
This product belongs to the following categories:
References- Koonin EV, Makarova KS, Rogozin IB, et al. The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple horizontal gene transfers. Genome Biol. 2003; 4:R19.
- Wang Y, Guan X, Fok KL, et al. A novel member of the rhomboid family, RHBDD1, regulates BIK-mediated apoptosis. Cell Mol. Life Sci. 2008; 65:3822-9.
- Wang Y, Song W, Li S, et al. GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules. BNC Cell Biol. 2009; 10:25.
Datasheet 10-01W
|
|
