|
Reagents
REDI-Ladder Molecular Weight Marker
Background
REDI-Ladder&trade prestained molecular weight marker contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. Since it is prestained it allows you to monitor molecular weight separation during electrophoresis, estimate the molecular weights of proteins of interest, and evaluate western blot transfer efficiency.
SPECIAL FEATURES:
1. Broad Range: 10-175 kDa
2. Ready-to-use: supplied in a loading buffer for direct loading on gels
3. Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with a blue dye
4. Sharp bands
|
Description
REDI-Ladder&trade Prestained Molecular Weight Marker run on a SDS-PAGE 15% Tris-Glycine gel.
|
Application
Overview:
1. Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
2. Monitoring of protein transfer onto membranes during western blotting
3. Sizing of proteins on SDS-polyacrylamide gels and western blots.
Recommendations for Loading:
1. Thaw the ladder either at room temperature or at 37-40 ˚C for a few minutes to dissolve any precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load 5 μl per well for mini gels, 2.5 μl per well for mini gel western blots, 10 μl per well for large gels and 5 μl per well for large gel western blots.
Tested Application
WB
Storage
Stable at 4˚C for 3 months, and -20 ˚C for 24 months.
Species Reactivity
n/a
Short Description
prestained molecular weight marker
This product belongs to the following categories:
Datasheet 10-01W
|
|
