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Primary Antibodies
Pyruvate Dehydrogenase Antibody
Background
Pyruvate Dehydrogenase Antibody.
Additional Names
PDHX, E3BP, OPDX, PDX1, proX, DLDBP, pyruvate dehydrogenase complex, component X, E3-binding protein, pyruvate dehydrogenase complex, lipoyl-containing component X
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Description
Left: Western blot analysis of pyruvate dehydrogenase in human pancreas lysate (35μg protein in RIPA buffer) using pyruvate dehydrogenase antibody (1μg/mL).
Below: Immunohistochemistry of pyruvate dehydrogenase in paraffin embedded human pancreas using pyruvate dehydrogenase antibody. A) Microwaved antigen retrieval with citrate buffer pH 6 (3μg/mL), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1μg/mL)
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Other Product Images
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Source
Pyruvate dehydrogenase antibody was raised against a synthetic peptide near the C-terminus of pyruvate dehydrogenase.
Purification
Pyruvate Dehydrogenase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Pyruvate Dehydrogenase antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1μg/ml. IHC: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3μg/ml.
Tested Application
E, WB, IHC
Buffer
Pyruvate dehydrogenase antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-107P - Pyruvate Dehydrogenase Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1307 - Human Pancreas Tissue Lysate
Species Reactivity
H, R, M, Chm
Protein Accession Number
NP_003468.1
Short Description
(CT) Pyruvate dehydrogenase / PDHX
This product belongs to the following categories:
Related Products
References- Li Y, Cao X, Li LX, Brubaker PL, Edlund H, Drucker DJ. {beta}-Cell Pdx1 Expression Is Essential for the Glucoregulatory, Proliferative, and Cytoprotective Actions of Glucagon-Like Peptide-1. Diabetes. 2005 Feb;54(2):482-91.
Datasheet 08-02W
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