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Primary Antibodies
Prosapip2 Antibody
Background
Prosapip2 (TBKBP1) is essential for signal transduction during viral infection thus plays a major role in the TNF/NF-kappaB pathway. It is an adaptor protein that constitutively binds TBK1 (TANK-binding kinase) and IKBKE and may play a role in antiviral innate immunity. Prosapip2 is a 615 amino acid adaptor protein belonging to the fibrillar collagen family, consisting of trimers of identical alpha 1 chains which are linked to each other by interchain disulfide bonds. It has a ubiquitous expression with highest levels in ovary, followed by the neuronal system. Prosapip2 binds to TBK1 and helps in the activation of IRF3 which controls the expression of antiviral genes during infection. Recent studies show that Prosapip2 is an interaction partner of ProSAP2/Shank3 and actin, suggesting a role as a linker molecule between postsynaptic density and the cytoskeleton.
Additional Names
Prosapip2 (CT), TANK-binding kinase 1-binding protein, TBK1-binding protein 1, TBKBP1, SINTBAD
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Description
Left: Western blot analysis of Prosapip2 in rat liver tissue lysate with Prosapip2 antibody at 1 μg/ml.
Below: Immunohistochemistry of Prosapip2 in rat liver tissue with Prosapip2 antibody at 5 μg/ml.
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Other Product Images
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Source
Prosapip2 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human Prosapip2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Prosapip2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Prosapip2 antibody can be used for detection of Prosapip2 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5397P - Prosapip2 Peptide
Storage
Prosapip2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1464 - Rat Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
7662302
Protein Accession Number
NP_055541
Short Description
TANK-binding kinase 1-binding protein
This product belongs to the following categories:
References- Bouwmeester T, Bauch A, Ruffner H, et al. A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nat. Cell Biol. 2004; 6:97–105
- Ryzhakov G and Randow F. SINTBAD, a novel component of innate antiviral immunity, shares a TBK1-binding domain with NAP1 and TANK. EMBO J. 2007; 26:3180–90.
- Meffert MK, Chang JM, Wiltgen BJ, et al. NF-kappa B functions in synaptic signaling and behavior. Nat. Neurosci. 2003; 6:1072–78.
- Liebau S, Proepper C, Schmidt T, et al. ProSAPiP2, a novel postsynaptic density protein that interacts with ProSAP2/Shank3. Biochem. Biophys. Res Commun. 2009; 385:460-5.
Datasheet 09-01W
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