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Primary Antibodies
Precerebellin Antibody
Background
Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q (1). Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway (2); and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release (3). The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus (4). Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin posseses functions other than those already stated (5).
Additional Names
Precerebellin (IN), Cerebellin precursor, Cbln1
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Description
Left: Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 μg/ml.
Below: Immunohistochemical staining of rat brain tissue using precerebellin antibody at 2 μg/ml.
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Other Product Images
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Source
Precerebellin antibody was raised against a 15 amino acid peptide from the middle of human precerebellin.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Precerebellin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Precerebellin / Cerebellin precursor / Cbln1 (Intermediate Domain) Peptide (Cat. No. 3589P)
Application
Precerebellin antibody can be used for the detection of precerebellin by Western blot at 2 - 4 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3589P - Precerebellin Peptide
Storage
Precerebellin antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1412 - Mouse Cerebellum Tissue Lysate
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
116114
Protein Accession Number
P23435
Short Description
(IN) Neuromodulatory protein precursor
This product belongs to the following categories:
Related Products
References- Urade Y, Oberdick J, Molinar-Rode R, et al. Precerebellin is a cerebellum-specific protein with similarity to the globular component C1q B chain. Proc. Natl. Acad. Sci. USA 1991; 88:1069-73.
- Mazzocchi G, Andreis PG, De Caro R, et al. Cerebellin enhances in vitro secretory activity of human adrenal gland. J. Clin. Endocrinol. Metab. 1999; 84:632-5.
- Albertin G, Malendowicz LK, Macchi C, et al. Cerebellin stimulates the secretory activity of the rat adrenal gland: in vitro and in vivo studies. Neuropeptides 2000; 34:7-11.
- Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Proc. Natl. Acad. Sci. USA 1985; 82:7145-8.
Datasheet 08-02W
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