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Primary Antibodies
Potassium Channel, Kv2.2 Subunit Antibody
Background
Voltage-gated K+ channels are important determinants of neuronal membrane excitability, and differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety on non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes. Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells.
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Description
Left: Potassium Channel, Kv2.2 Subunit Antibody
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Purification
Affinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
Potassium Channel antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Rabbit anti-Potassium Channel, Kv2.2 Subunit polyclonal antibody was raised against a synthetic peptide specific for the Kv2.2 subunit in Xenopus and rodent conjugated to keyhole limpet hemocyanin (KLH). Rabbit anti-Potassium Channel, Kv2.2 Subunit was prepared from rabbit serum by affinity purification using a Sulfo-Link column matrix to which the peptide immunogen was coupled.
Application
Potassium Channel antibody can be used for specific immunolabeling of the ~125k Kv 2.2 protein in Western blots. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). Rabbit anti-Potassium Channel, Kv2.2 recognizes Kv2.2 in rat and Xenopus. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.
Tested Application
WB, IHC
Buffer
Potassium Channel antibody is supplied as affinity purified polyclonal antibody, 10 μg antibody vial; lyophilized from ammonium bicarbonate (5mM) so some residual salt may also be present. The antibody should be reconstituted in 50 μl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. There is adequate amount of material to conduct 10-mini Western Blots.
Storage
Potassium Channel, Kv2.2 Subunit antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C and is stable at -20˚C for at least 1 year. Ambient shipment is suitable for both international and domestic.
Species Reactivity
R, X
Short Description
Potassium Channel, Voltage Gated, Kv2.2 Subunit Pan-Specific
This product belongs to the following categories:
References- Burger, C. and Ribera, A.B. “Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development,” J Neurosci. 16 (1996) 1412 - 1421.
- Pongs, O. “Voltage-gated potassium channels: from hyperexcitability to excitement,” FEBS Lett. 452 (1999) 31 – 35.
- Maletic-Savatic, M., Lenn, N.J., and Trimmer, J.S. “Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro,” J Neurosci. 15 (1995) 3840 – 3851.
- Hwang, P.M., Fotuhi, M., Bredt, D.S., Cunningham, A.M., and Snyder, S.H. “Contrasting immunohistochemical localizations in rat brain of two novel K+ channels of the Shab subfamily,” J. Neurosci., 13 (1993) 1569 - 1576.
Datasheet 0802-W
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