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Primary Antibodies
PTK7 Antibody
Background
Protein-tyrosine kinases (PTKs) play important roles in regulating cell proliferation and differentiation during development. One member of the PTK family, PTK7, has been suggested to regulate the planar cell polarity (PCP) pathway in vertebrates and may play a role in neural convergent extension and neural tube closure. PTK7 has also been implicated in the development of cancer. Loss of PTK7 expression was seen in several melanoma cell lines and biopsies. Conversely, high-throughput analysis of acute myeloid leukemia samples showed an increased level of PTK7 expression compared to normal bone marrow and purified CD34+ cells. Multiple isoforms of PTK7 are known to exist.
Additional Names
PTK7 (NT), Protein tyrosine Kinase 7, colon carcinoma kinase-4, CCK-4
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Description
Left: Western blot analysis of PTK7 in (A) human, (B) mouse and (C) rat colon tissue lysate with PTK7 antibody at 1 μg/ml.
Below: Immunohistochemistry of PTK7 in human colon tissue with PTK7 antibody at 5 μg/ml.
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Other Product Images
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Source
PTK7 antibody was raised against a 15 amino acid peptide from near the amino terminus of human PTK7.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PTK7 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PTK7 antibody can be used for detection of PTK7 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4299P - PTK7 Peptide
Storage
PTK7 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1320 - Human Colon Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
47938093
Protein Accession Number
AAH71557
Short Description
(NT) Protein tyrosine kinsase 7
This product belongs to the following categories:
Related Products
References- Park SK, Lee HS and Lee ST. Characterization of the human full-length PTK7 cDNA encoding a receptor protein kinase-like molecule closely related to chick KLG. J. Biochem. 1996; 119:235-9.
- Lu X, Borchers AG, Jolicoeur C, et al. PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates. Nature 2004; 430:93-8.
- Easty DJ, Mitchell PJ, Patel K, et al. Loss of expression of receptor tyrosine kinase family genes PTK7 and SEK in metastatic melanoma. Int. J. Cancer 1997; 1061-5.
- Muller-Tidow C, Schwable J, Steffen B, et al. High-throughput analysis of genome-wide receptor tyrosine kinase expression in human cancers identifies potential novel drug targets. Clin. Cancer Res. 2004; 10:1241-9.
Datasheet 08-03W
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