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Primary Antibodies
PLKK (Phospho-Ser482,486,490) Antibody
Background
Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
Purification
Affinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
PLKK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Rabbit anti-PLKK (Ser482/486/490) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus Phospho-Ser482,486,490 PLKK. The antigen sequence used is closely homologous to rodent and human forms of PLKK. Rabbit anti-PLKK (Ser482/486/490) was purified by sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Application
PLKK antibody is specific for PLKK phosphorylated at Ser482,486,490. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Xenopus is the only species tested to date. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Tested Application
WB
Buffer
PLKK antibody is supplied as affinity purified polyclonal antibody, 100 μl in 150 mM NaCl, 10 mM HEPES, 100 μg per ml BSA and 50% glycerol, pH 7.5., and there is adequate amount of material to conduct 10-mini Western Blots.
Storage
For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Species Reactivity
X
This product belongs to the following categories:
References- van Vugt, M.A.T.M., van de Weerdt, B.C.M., Vader, G., Janssen, H., Calafat, J., Klompmaker, R., Wolthuis, R.M.F., and Medema, R.H., “Polo-like Kinase-1 Is Required for Bipolar Spindle Formation but Is Dispensable for Anaphase Promoting Complex/Cdc20 Activation and Initiation of Cytokinesis,” J. Biol. Chem., 279 (2004) 36,841 – 36,854.
- Erikson, E., Haystead, T.A.J., Qian, Y-W., and Maller, J.L., “A Feedback Loop in the Polo-like Kinase Activation Pathway,” J. Biol. Chem., 279 (2004) 32,219-32,224.
- Liu, J., Lewellyn, A.L., Chen, L.G., and Maller, J.L., “The Polo Box Is Required for Multiple Functions of Plx1 in Mitosis,” J. Biol. Chem., 279 (2004) 21,367 - 21,373.
- Kumagai, A., and Dunphy, W. G., “Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts,” Science 273 (1996) 1377 – 1380.
Datasheet 0802-W
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