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Primary Antibodies
PLEKHM3 Antibody
Background
PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.
Additional Names
PLEKHM3, Pleckstrin homology domain containing family M member 3, PLEKHM1L, DAPR
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Description
Left: Western blot analysis of PLEKHM3 in mouse skeletal muscle tissue lysate with PLEKHM3 antibody at (A) 1 and (B) 2 μg/ml.
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Source
PLEKHM3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human PLEKHM3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PLEKHM3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5487P - PLEKHM3 Peptide
Storage
PLEKHM3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
172046173
Protein Accession Number
Q6ZWE6
Short Description
Pleckstrin homology domain containing family M member 3
This product belongs to the following categories:
References- Virtanen C, Paris J, and Takahashi M. Identification and characterization of a novel gene, dapr, involved in skeletal muscle differentiation and protein kinase B signaling. J. Biol. Chem. 2009; 284:1636-43.
Datasheet 10-01W
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