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Primary Antibodies
PHAP Antibody
Background
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Additional Names
PHAP (CT), PHAP
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Description
Left: Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 μg/ml. The two bands Below: PHAP I might be differently spliced isoforms of PHAP.
Below: Immunohistochemistry of PHAP in human small intestine tissue with PHAP antibody at 10 μg/ml.
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Other Product Images
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Source
PHAP antibody was raised with a synthetic peptide corresponding to amino acids at carboxy terminus of human PHAP I.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PHAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human PHAP (C-Terminus) Peptide (Cat. No. 3152P)
Application
PHAP antibody can be used for detection of PHAP I and PHAP III by Western blot at 0.5 to 2 μg/ml. At approximately 35 kDa and 32 kDa a band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3152P - PHAP Peptide
Storage
PHAP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1207 - Raji Cell Lysate
- Cat. No. 1308 - Human Small Intestine Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
730318
Protein Accession Number
P39687
Short Description
(CT) Regulator of mitochondrion apoptosis
This product belongs to the following categories:
Related Products
References- Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-α in a death regulatory pathway. Science. 2003;299(5604):223-6.
- Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.
Datasheet 08-02W
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