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Primary Antibodies
PERP Antibody
Background
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6), MTBP (7) and PERP (8), were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein (8-10). p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis (8). The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family (8).
Additional Names
PERP
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Description
Left: Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with PERP antibody at 1 μg /ml.
Below: Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 μg/ml.
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Other Product Images
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Source
PERP antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human PERP, which differ from the mouse sequence by three amino acids (7-9) .
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
PERP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human PERP Peptide (Cat. No. 2451P)
Application
PERP antibody can be used for detection of PERP by Western blot at 0.5 to 1 μg/ml. A431 cell lysate can be used as positive control and a 21 kDa band can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2451P - PERP Peptide
Storage
PERP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
Species Reactivity
H
Protein GI Number
11528520
Protein Accession Number
np_071315
Short Description
A mediator of p53 induced apoptosis
This product belongs to the following categories:
Related Products
References- Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.
- Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9.
- Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8.
- Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.
Datasheet 08-02W
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