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Primary Antibodies
PDL-1 Antibody
Background
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2.
Additional Names
PDL-1, Programmed cell death 1 ligand-1, programmed death ligand 1, PD-L1, B7-H1
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Description
Left: Western blot analysis of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemistry of PDL-1 in human heart tissue with PDL-1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
PDL-1 antibody was raised against a 17 amino acid peptide from near the center of human PDL-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PDL-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PDL-1 antibody can be used for detection of PDL-1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4059P - PDL-1 Peptide
Storage
PDL-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1207 - Raji Cell Lysate
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
7661534
Protein Accession Number
NP_054862
Short Description
A novel ligand of PDCD-1
This product belongs to the following categories:
Related Products
References- Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Datasheet 08-02W
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