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Primary Antibodies
PD-L1 Antibody
Background
PD-L1 Antibody.
Additional Names
CD274 antigen, PDCD1LG1, B7-H, B7H1, PDL1, PD-L1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635
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Description
Left: Western blot analysis of PD-L1 in human heart lysate (RIPA buffer, 30μg total protein per lane) using PD-L1 antibody (0.3μg/mL).
Below: PD-L1 staining of differentiated human skeletal muscle PD-L1 antibody (4μg/mL).
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Other Product Images
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Source
PD-L1 antibody was raised against a synthetic peptide of PD-L1.
Purification
PD-L1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
PD-L1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 35kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). Recommended concentration: 0.3-1.0μg/ml. Immunohistochemistry: In paraffin embedded Human Skel;etal Muscle shows staining of the striated muscular fibres. Recommended concentration, 4-6μg/ml.
Tested Application
E, WB, IHC
Buffer
PD-L1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-156P - PD-L1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_054862.1
Short Description
CD274 / PD-L1
This product belongs to the following categories:
Related Products
References- Thompson RH, Gillett MD, Cheville JC, Lohse CM, Dong H, Webster WS, Krejci KG, Lobo JR, Sengupta S, Chen L, Zincke H, Blute ML, Strome SE, Leibovich BC, Kwon ED. Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17174-9. Epub 2004 Dec 7.
Datasheet 08-01W
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