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Recombinant Proteins
PD-1 Recombinant Protein
Background
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of “danger signals” such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.
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Description
Left: SDS-PAGE analysis of recombinant PD-1 on Coomassie Blue-stained 12% SDS-PAGE gel.
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Source
E. coli
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Tested Application
E, WB, MS
Buffer
1X PBS containing 0.1% SDS
Storage
Store at -70˚C. As with any protein, exposing PD-1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Protein GI Number
148707975
Protein Accession Number
EDL39922
Domain Information
aa 22 - 170
Molecular Weight
19 kDa (Calculated)
Fusion Partner
C-terminal His-tag
Purity
~95%
Short Description
E. coli expressed Mouse PD-1
This product belongs to the following categories:
References- Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- Ishida Y, Agata Y, Shibahara K, et al. Induced expression of EPAC1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
- Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.
Datasheet 09-01W
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