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Primary Antibodies
PD-1 Antibody
Background
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of “danger signals” such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
Additional Names
PD-1 (CT), Programmed cell death 1, PDCD1, CD279
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Description
Left: Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
Below: Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
PD-1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human PD-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PD-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PD-1 antibody can be used for detection of PD-1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4065P - PD-1 Peptide
Storage
PD-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1208 - THP-1 Cell Lysate
Species Reactivity
H, M
Protein GI Number
145559515
Protein Accession Number
Q15116
Short Description
(CT) Programmed cell death 1 protein
This product belongs to the following categories:
Related Products
References- Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
- Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.
Datasheet 08-03W
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