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46-139


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

PAX6 Antibody

Background

PAX6 Antibody. This antibody is expected to recognize both reported isoforms (NP_000271.1 and NP_001595.2).

Additional Names

PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6

Source

PAX6 antibody was raised against a synthetic peptide near the C-terminus of PAX6.

Purification

PAX6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

PAX6 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments in human and mouse brain lysates gave no specific signal but low background (at antibody concentration up to 1μg/ml).

Tested Application

E

Buffer

PAX6 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Species Reactivity

H, M, R

Protein Accession Number

NP_000271.1, NP_001595.2

Short Description

(CT) PAX6

This product belongs to the following categories:

Related Products

References

  1. Grinchuk O, Kozmik Z, Wu X, Tomarev S. The Optimedin gene is a downstream target of Pax6. J Biol Chem. 2005 Oct 21;280(42):35228-37. Epub 2005 Aug 22.


Datasheet 08-01W

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