|
Primary Antibodies
PAK2 Antibody
Background
The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.
Additional Names
PAK2 (NT), p21-activated kinase 2, PAK65
|
Description
Left: Western blot analysis of PAK2 in Jurkat lysate with PAK2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of PAK2 in mouse spleen tissue with PAK2 antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
PAK2 antibody was raised against a 14 amino acid peptide from near the amino terminus of human PAK2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PAK2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PAK2 antibody can be used for the detection of PAK2 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3887P - PAK2 Peptide
Storage
PAK2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1406 - Mouse Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
32483399
Protein Accession Number
NP_002568
Short Description
(NT) serine-threonine kinase, cdc42, Rac
This product belongs to the following categories:
Related Products
References- Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.
- Rudel T and Bokoch GM. Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2. Science 1997; 276:1571-4.
- Vilas GL, Corvi MM, Plummer GJ, et al. Posttranslational myristoylation of caspase-activated p21-activated protein kinase 2 (PAK2) potentiates late apoptotic events. Proc. Natl. Acad. Sci. USA 2006; 103:6542-7.
Datasheet 08-02W
|
|
