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Primary Antibodies
PAI-1 Antibody
Background
PAI-1 Antibody.
Additional Names
SERPINE1, PAI, PAI1, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I
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Description
Left: Western blot analysis of PAI-1 in human liver lysate (35μg protein in RIPA buffer) using PAI-1 antibody (0.1μg/mL).
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Source
PAI-1 antibody was raised against a synthetic peptide at an internal region of PAI-1.
Purification
PAI-1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
PAI-1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx 45kDa band observed in human duodenum, liver and lung lysates (calculated MW of 45.1kDa according to NP_000593.1). Recommended concentration: 0.1-0.3μg/ml.
Tested Application
E, WB
Buffer
PAI-1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-142P - PAI-1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity
H, D, Chm
Protein Accession Number
NP_000593.1
Short Description
(IN) PAI-1 / SERPINE1
This product belongs to the following categories:
Related Products
References- Baumruker T, Csonga R, Pursch E, Pfeffer A, Urtz N, Sutton S, Bofill-Cardona E, Cooke M, Prieschl E. Activation of mast cells by incorporation of cholesterol into rafts. Int Immunol. 2003 Oct;15(10):1207-18.
Datasheet 08-02W
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