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Primary Antibodies
PACSIN3 Antibody
Background
PACSIN3 Antibody.
Additional Names
PACSIN3, protein kinase C and casein kinase substrate in neurons 3, SH3 domain-containing protein 6511, SDPIII, syndapin III
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Description
Left: Western blot analysis of PACSIN3 in H460 lysate (RIPA buffer, 35μg total protein per lane) using PACSIN3 antibody (1μg/mL).
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Source
PACSIN3 antibody was raised against a synthetic peptide of PACSIN3.
Purification
PACSIN3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
PACSIN3 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in H460 and human heart and muscle lysates (predicted MW of 49kDa according to NP_057307.2). Recommended for use at 1-3μg/ml.
Tested Application
E, WB
Buffer
PACSIN3 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M, R
Protein Accession Number
NP_057307.2
Short Description
PACSIN3
This product belongs to the following categories:
Related Products
References- Cuajungco MP, Grimm C, Oshima K, D'hoedt D, Nilius B, Mensenkamp AR, Bindels RJ, Plomann M, Heller S. PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4. J Biol Chem. 2006 Jul 7;281(27):18753-62. Epub 2006 Apr 20.
Datasheet 08-01W
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