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Primary Antibodies
Osteopontin Antibody
Background
Osteopontin (OPN) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1. Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells. It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.
Additional Names
SPP1, BNSP, BSPI, CIT:779436, ETA-1, MGC110940, NEPHROPONTIN, OPN, SPP-1, UROPONTIN, Bone Sialoprotein 1, eta-1, osteopontin, uropontin
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Description
Left: Immunohistochemistry staining of Osteopontin in brain, cortex (formalin-fixed paraffin-embedded) using Osteopontin antibody.
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Purification
Sterile Filtration
Clonality / Clone
This is a polyclonal antibody.
Host
Osteopontin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Osteopontin antibody was raised against a synthetic peptide of Osteopontin conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. (Human)
Application
Osteopontin antibody can be used for detection of Osteopontin by WB (1:500 - 1:2000), ELISA (1:20000 - 1:100000), IHC-parafin (1:500).
Tested Application
E, WB, IHC
Buffer
Osteopontin antibody is supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
Storage
Osteopontin antibody can be stored at 4˚C or -20˚C. Avoid repeated freezing and thawing.
Species Reactivity
D, P
Formulation
Volume: 50 μl
Concentration: 85 mg/ml
Short Description
Osteopontin
This product belongs to the following categories:
Datasheet 09-01W
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