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Primary Antibodies
Osteopontin Antibody
Background
Osteopontin Antibody.
Additional Names
SPP1, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), HGNC:11255, BNSP, BSPI, ETA-1, MGC110940, OPN , Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1
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Description
Left: Western blot analysis of osteopontin in MOLT4 cell lysate (RIPA buffer, 30μg total protein per lane) using osteopontin antibody (1μg/mL).
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Source
Osteopontin antibody was raised against a synthetic peptide near the C-terminus of SPP1.
Purification
Osteopontin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Osteopontin antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 1-3μg/ml.
Tested Application
E, WB
Buffer
Osteopontin antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-152P - Osteopontin Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1206 - MOLT4 Cell Lysate
Species Reactivity
H, M, R, D
Protein Accession Number
NP_000573.1
Short Description
(CT) Osteopontin / SPP1
This product belongs to the following categories:
Related Products
References- Kolb A, Kleeff J, Guweidhi A, Esposito I, Giese NA, Adwan H, Giese T, Buchler MW, Berger MR, Friess H. Osteopontin Influences the Invasiveness of Pancreatic Cancer Cells and is Increased in Neoplastic and Inflammatory Conditions. Cancer Biol Ther. 2005 Jul 5;4(7) [Epub ahead of print]
Datasheet 08-02W
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