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Primary Antibodies
OTUD5 Antibody
Background
OTUD5 is a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and OTUD5 has been shown to suppress the type I interferon (IFN-I)-dependent innate immune response by cleaving the polyubiquitin chain from TRAF3, an essential type I interferon adaptor protein. Cleavage results in disassociation of TRAF3 from a downstream signaling complex containing TBK1 and the disruption of the IFN-I signaling cascade, indicating that OTUD5 acts as a negative regulator of innate immune responses. It has been suggested that by suppressing IFN-I production, OTUD5 may function to inhibit the emergence of certain autoimmune disorders such as systemic lupus erythematosus. Multiple isoforms of OTUD5 are known to exist.
Additional Names
OTUD5, OTU domain-containing protein 5, Deubiquitinating enzyme A, DUBA
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Description
Left: Western blot analysis of OTUD5 in human kidney lysate with OTUD5 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of OTUD5 in mouse kidney tissue with OTUD5 antibody at 2.5 μg/ml.
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Other Product Images
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Source
OTUD5 antibody was raised against a 13 amino acid peptide near the carboxy terminus of the human OTUD5.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
OTUD5 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
OTUD5 antibody can be used for detection of OTUD5 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4811P - OTUD5 Peptide
Storage
OTUD5 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
119571108
Protein Accession Number
EAW50723
Short Description
OTU domain-containing protein 5
This product belongs to the following categories:
Related Products
References- Kayagi N, Phung Q, Chan S, et al. DUBA: A deubiquitinase that regultes type I interferon production. Science 2007; 318:1628-32.
- Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59.
Datasheet 08-01W
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