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Primary Antibodies
ORAI3 Antibody
Background
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.
Additional Names
ORAI3 (CT), Transmembrane protein 142C, TMEM142C, Calcium release-activated ORAI3 (CT), Transmembrane protein 142C, TMEM142C, Calcium release-activated
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Description
Left: Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 μg/ml.
Below: Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 μg/ml.
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Other Product Images
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Source
ORAI3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ORAI3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ORAI3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 – 4 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4215P - ORAI3 Peptide
Storage
ORAI3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1288 - A20 Cell Lysate
Species Reactivity
H, M
Protein GI Number
74732916
Protein Accession Number
Q9BRQ5
Short Description
(CT) a T cell calcium channel
This product belongs to the following categories:
Related Products
References- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
- Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.
Datasheet 08-02W
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