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Primary Antibodies
ORAI2 Antibody
Background
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.
Additional Names
ORAI2, Transmembrane protein 142B, TMEM142B, Calcium release-activated calcium channel protein 2
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Description
Left: Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 μg/ml.
Below: Immunocytochemistry of ORAI2 in Jurkat cells with ORAI2 antibody at 5 μg/ml.
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Other Product Images
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Source
ORAI2 antibody was raised against an 15 amino acid peptide from near the carboxy terminus of human ORAI2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ORAI2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4111P - ORAI2 Peptide
Storage
ORAI2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M
Protein GI Number
74732728
Protein Accession Number
Q96SN7
Short Description
(CT) a T cell calcium channel
This product belongs to the following categories:
Related Products
References- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
- Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.
Datasheet 08-02W
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