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Primary Antibodies
ORAI1 Antibody
Background
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Additional Names
ORAI1 (NT), Transmembrane protein 142A, TMEM142A, Calcium release-activated calcium channel protein 1
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Description
Left: Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 μg/ml in the (A) absence or (B) presence of blocking peptide.
Below: Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 μg/ml.
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Other Product Images
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Source
ORAI1 antibody was raised against an 18 amino acid peptide from near the amino terminus of human ORAI1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ORAI1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
ORAI1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1316 - Human Ovary Tissue Lysate
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M
Protein GI Number
97180269
Protein Accession Number
Q96D31
This product belongs to the following categories:
Related Products
References- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
Datasheet 08-02W
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