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Detection Sets
ORAI Detection Set
Background
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. The ORAI family is a recently identified set of proteins that are essential components of these CRAC channels. A missense mutation in the ORAI1 protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with the stromal interaction molecule 1 (STIM1), the store-activated endoplasmic reticulum Ca++ sensor. Like ORAI1, ORAI2 also functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, although at a lesser efficacy than ORAI1. Although ORAI3 can also function as Ca++ plasma membrane channel, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. STIM1, in its function as a Ca++ sensor and an activator of CRAC channels, migrates to the plasma membrane from endoplasmic reticulum-like sites which act as cellular Ca++ stores. A related molecule, STIM2, inhibits the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting these two proteins may play a coordinated role in controlling Ca++ entry. The ORAI antibodies are predicted to have no cross-reactivity to the other ORAI proteins. Similarly, the STIM antibodies will not cross-react with the other STIM protein.
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Description
For images please see PDF data sheet
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Kit Contents
ORAI1 Antibody, Catalog No. 4041 (50μg)
ORAI2 Antibody, Catalog No. 4111 (50μg)
ORAI3 Antibody (CT), Catalog No. 4215 (50μg)
STIM1 Antibody, Catalog No. 4119 (50μg)
STIM2 Antibody (CT), Catalog No. 4123 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of Bonzo, CCR3, CCR5, CCR8, Cx3CR1, CXCR4, DC-SIGN and GPR15 by immunoblot at 0.5 – 2 μg/ml. Some of these antibodies can also be used at 1 – 20 μg/ml to detect their respective proteins via immunohistochemistry or immunocytochemistry.
Tested Application
WB
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Positive Control
- ORAI1 Antibody: Human Ovary Tissue Lysate, Catalog No. 1316
ORAI2 Antibody: Jurkat Cell Lysate, Catalog No. 1205
ORAI3 Antibody: A-20 Cell Lysate, Catalog No. 1288
STIM1 Antibody: Mouse Thymus Tissue Lysate, Catalog No. 1409
STIM2 Antibody: A-20 Cell Lysate, Catalog No. 1288
Species Reactivity
see datasheet
This product belongs to the following categories:
Related Products
References- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
Datasheet 0803-W
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