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Primary Antibodies
OMI Antibody
Background
Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication (1). Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases (reviewed in 2). The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs (reviewed in 3). Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs (4). Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis (5).
Additional Names
OMI (C2), HtrA2
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Description
Left: Western blot analysis of OMI in U937 lysate with Omi antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunohistochemistry of OMI in human liver tissue with OMI antibody at 2 μg/ml.
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Other Product Images
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Source
OMI antibody was raised against a peptide corresponding to 16 amino acids near the C-terminus of human Omi.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
OMI antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human OMI / HtrA2 (C-Terminus 2) Peptide (Cat. No. 3051P)
Application
OMI antibody can be used for detection of OMI by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3051P - OMI Peptide
Storage
OMI antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1215 - U937 Cell Lysate
Species Reactivity
H
Protein GI Number
5870865
Protein Accession Number
AAB94569
Short Description
(C2) IAP inhibitor
This product belongs to the following categories:
Related Products
References- Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174.
- Liston P, Fong WG, and Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80.
- Vaux DL and Silke J. Mammalian mitochondrial IAP binding proteins. Biochem. Biophys. Res. Comm. 2003; 304:499-504.
- Suzuki Y, Imai Y, Nakayama H, et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 2001; 8:613-21.
Datasheet 08-02W
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