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Primary Antibodies
OCLN Antibody
Background
Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.
Additional Names
OCLN, Occludin
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Description
Left: Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 μg/ml.
Below: Immunohistochemistry of OCLN in human liver tissue with OCLN antibody at 2.5 μg/ml.
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Other Product Images
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Source
OCLN antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human OCLN.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
OCLN antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
OCLN antibody can be used for detection of OCLN by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5191P - OCLN Peptide
Storage
OCLN antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
20987418
Protein Accession Number
AAH29886
Short Description
a component of tight junction strands
This product belongs to the following categories:
References- Tsukita S, Furuse M, and Itoh M. Multifunctional strands in tight junctions. Nat. Rev. Mol. Cell Biol. 2001; 2:285-93.
- Chiba H, Osanai M, Murata M, et al. Transmembrane proteins of tight junctions. Biochim. Biophys. Acta 2008; 1778:588-600.
- Furuse M, Hirase T, Itoh M, et al. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol. 1993; 1777-88.
- Matter K, Aijaz S, Tsapara A, et al. Mammalian tight junctions in the regulation of epithelial differentiation and proliferation. Curr. Opin. Cell Biol. 2005; 17:453-8.
Datasheet 09-01W
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