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Primary Antibodies
Noxa Antibody
Background
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified , which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 . Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3). Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4).
Additional Names
Noxa, PMA-induced protein 1, PMAIP1, APR
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Description
Left: Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 μg/ml.
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Other Product Images
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Source
Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.
Purification
Immunoaffinity chromatography purified IgG
Clonality / Clone
This is a polyclonal antibody.
Host
Noxa antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Noxa Peptide (Cat. No. 2437P)
Application
Noxa antibody can be used for detection of Noxa by Western blot at 0.5 to 1 μg/ml. Human stomach cell lysate can be used as a positive control. Noxa antibody is human, mouse and rat reactive. This product is for research use only.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2437P - Noxa Peptide
Storage
Noxa antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1319 - Human Stomach Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
10946832
Protein Accession Number
NP_067426
Short Description
A p53 target gene
This product belongs to the following categories:
Related Products
References- Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
- Nakano K and Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 2001; 7:683-94.
- Yu J, Zhang L, Hwang PM, et al. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 2001; 7:673-82.
- Eferl R, Ricci R, Kenner L, et al. Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53. Cell 2003; 112:181-92.
Datasheet 08-02W
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