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Primary Antibodies
Nogo 66 Receptor Antibody
Background
Nogo 66 Receptor Antibody.
Additional Names
RTN4R, reticulon 4 receptor, HGNC:1860, NGR, NOGOR, Nogo-66 receptor, UNQ330/PRO526, nogo receptor, reticulon 4 receptor precursor
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Description
Left: Western blot analysis of Nogo 66 Receptor in human brain lysate (35μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide using Nogo 66 Receptor antibody (0.3μg/mL).
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Source
Nogo 66 Receptor antibody was raised against a synthetic peptide of Nogo 66 Receptor.
Purification
Nogo 66 Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Nogo 66 Receptor antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:16,000. Western Blot: experiments gave bands at approx 90-100kDa and 37+40+45kDa in human brain lysate after 0.3μg/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1. Recommended concentration: 0.3-1μg/ml.
Tested Application
E, WB
Buffer
Nogo 66 Receptor antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R, D
Protein Accession Number
NP_075380.1
Short Description
Nogo 66 receptor
This product belongs to the following categories:
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References- Shao Z, Browning JL, Lee X, Scott ML, Shulga-Morskaya S, Allaire N, Thill G, Levesque M, Sah D, McCoy JM, Murray B, Jung V, Pepinsky RB, Mi S. TAJ/TROY, an orphan TNF receptor family member, binds Nogo-66 receptor 1 and regulates axonal regeneration. Neuron. 2005 Feb 3;45(3):353-9.
Datasheet 08-01W
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