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Primary Antibodies
Nhe-1 Antibody
Background
The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
Additional Names
Nhe-1 (CT), Sodium-hydrogen exchanger 1, solute carrier family 9 member 1, SLC9A1, Na+/H+ antiporter
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Description
Left: Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 μg/ml.
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Source
Nhe-1 antibody was raised against a 20 amino acid peptide near the carboxy terminus of the human Nhe-1.
Clonality / Clone
This is a polyclonal antibody.
Host
Nhe-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 – 2 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
Nhe-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1465 - Rat Kidney Tissue Lysate
Species Reactivity
H,M,R
Protein Accession Number
P19634
Data Sheet Version
08-02W
Related Products
References
- Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand. 1987; 76:545-7.
- Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell. 2000; 6:1425-36.
- Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+)/H(+) exchange inhibition. Hypertension 2003; 41:373-7.
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